Coding

Part:BBa_K2201260:Design

Designed by: Svenja Vinke   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and mRFP fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2078
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1521
    Illegal BamHI site found at 2202
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 912
    Illegal AgeI site found at 1024
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mRFP CDS is fused to the CDS of the little subunit via gibson assembly.


Source

E_1010 (mRFP) and BBa_K1465202 (RuBisCo).

References